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Inoculating test suspension onto metal disc.

EN 13697:2015

Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of disinfectants used in food, industrial, domestic and institutional areas.

EN 13697 is a phase 2 step 2 carrier test performed on surface disinfectants intended for use in the food, industrial, domestic and institutional areas without mechanical action to test the efficacy of the product against bacteria and/or fungi.

Yeast and fungi occur naturally in the environment. Although many assume both microorganisms are the same, they are not. Yeast is a single-celled microorganism and it reproduces through mitosis. Fungus however, is a multi-celled microorganism that reproduces through spores. Efficacy against yeast is a mandatory requirement according to the European Norms.

 

Test Conditions


The standard refers to the parameters to be observed when testing products intended for surface disinfection without mechanical action. This includes the test microorganism, test temperature, carrier, contact time and interfering substance. 

Refer to the table below for the minimum requirements for yeasticidal and fungicidal activity testing.

 

Test Method

 

In a carrier test, the test microorganism is added to the selected interfering substance and applied onto a metal disc. The metal disc represents an actual instrument or carrier of microorganism. The disc is then left to air-dry to simulate surface or carrier of microorganism. Next, test disinfectant is applied to the inoculated area for the duration of the contact time. The carrier is then submerged into a neutralizer solution to prevent continued activity. A sample of the neutralizer solution is then acquired, plated and incubated for 3 days. The number of test microorganism recovered is compared to the microorganism recovered from the control sample (where test microorganism is exposed to water instead of disinfectant) to determine if the test product is effective.

Diagram 1: EN 13697 test method

 

Log Reduction

 

Log reduction refers to the extent to which a product is capable of reducing the number of microbes. For example, 4-log reduction means the number of microorganisms on a surface has been reduced by 10 000 times. A product that is 99.9% effective against a certain microbe is said to have achieved 3-log reduction against that microbe.

For a product to pass EN 13697, it must be able to achieve 3-log reduction against the respective test microorganisms listed in Table 1 for yeasticidal and fungicidal activities. In other words, the product must be able to kill 99.9% yeasts and fungi while meeting all the other requirements of the European standard.

Difference between test product that pass & fail EN 13697 for A. brasiliensis

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